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  • Rapid detection of E. coli cells in urine samples using a ...

    Escherichia coli is one of the main causes of urinary tract infections (UTIs). E. coli is commonly detected from urine using standard culture method. However, the urine sampling and analysis required for these methods can be costly, time consuming (requires 24 to 48 hours) and labor-intensive. This …

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  • A multiplex PCR method to detect 14 Escherichia coli ...

    E. coli strains belonging to 14 serogroups, including O1, O2, O4, O6, O7, O8, O15, O16, O18, O21, O22, O25, O75 and O83, are the most frequently detected UPEC strains in a diverse range of clinical urine specimens. In the current study, the O-antigen gene clusters of E. coli serogroups O1, O2, O18 and O75 were characterized.

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  • Urine Culture with E. Coli: Meaning, Results and

    2021-6-23 · The presence of E. coli in urine usually indicates a urinary tract infection, but it may not always cause visible symptoms. When symptoms appear, they may include a burning sensation while urinating, a strong urge to urinate, passing small amounts of urine, passing strong-smelling urine, and urine that appears cloudy or red.

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  • Sensitive and rapid detection of pathogenic bacteria

    To test our chip, bacteria (E. coli, S. aureus, S. typhimurium, P. mirabilis, and P. aeruginosa) were spiked in the sterile urine to varying final concentrations (10 3, 10 4, 10 5, and 10 6 CFU mL −1) to simulate real UTI urine samples. For detection, bacteria in urine samples were enriched by a home-made pipette to improve the sensitivity and practicability of our centrifugal chip.

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  • Rapid identification of urinary tract infections based

    2019-11-1 · Detection of bacteria in clinical urine samples. Calibration curves for detection of E. coli (a) and S. aureus (b) in urine. The red and blue curves represents the signal for the detection of bacteria with or without Pt NP probes amplification, respectively. (c) Three-dimensional histogram of selectivity of capture probes to E. coli and S

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  • E. Coli and UTIs (Urinary Tract Infections): The

    Researchers at McMaster University have now developed a paper strip test that is cheap to produce, extremely portable, simple to use, and detects E. coli in water in 30 minutes.

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  • New paper strip test detects E. coli quickly, cheaply

    2019-12-1 · In this study we present a sensitive and rapid optical sandwich fluorescent immunoassay able to detect and quantify E. coli K12 in less than 25 min in synthetic urine without the need of sample preparation, with the assistance of a smartphone camera. The new sandwich immunossay presents several advantages, such as: 1) affordable off-the-shelf immunoassay reagents, 2) rapid procedure …

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  • Microfluidic smartphone quantitation of Escherichia

    2016-6-15 · A paper-based ELISA has been developed recently that can detect E. coli or bacteriuria in 5 h (Shih et al., 2015). However, the disadvantages include less sample volume of the microtitration plate, long incubation time required for each step ( Ivnitski et al., 1999 ), requires extensive sample cleaning and biomolecule purification and are label dependent ( Skottrup et al., 2008 ).

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  • Recent advances in biosensor based diagnosis of

    2021-4-13 · E.Coli & Coliforms Chromogenic Medium is used for fast, accurate simultaneous detection of E. coli and Coliform bacteria, incubated for 24 hours, E. coli was blue-green - purple, coliform is red. Ingredients (g/L): Special Nutrients. 39.0.

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  • Researchers Develop Rapid Test Strips for E. coli -

    New paper strips detect Escherichia coli simply, cheaply and quickly. 2 2. The E. coli-detecting paper strips developed at McMaster University (Photo: JD Howell) Nothing can put a dampener on a ...

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  • COLIKAT RAPID - Enumeration of Escherichia coli and ...

    Escherichia coli (E. coli) bacteria normally live in the intestines of people and animals. Most E. coli are harmless and actually are an important part of a healthy human intestinal tract. However, some E. coli are pathogenic, meaning they can cause illness, either diarrhea or illness outside of the intestinal tract. The types of E. coli that can cause diarrhea can be transmitted through ...

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  • New paper strip test detects E. coli quickly, cheaply

    2021-4-13 · E.Coli & Coliforms Chromogenic Mediumis the culture medium of Qingdao haibo biological supply.Provide detailed information about E.Coli & Coliforms Chromogenic Medium formulation, price, specification and usage. Product number Product name Product

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  • Questions and Answers | E. coli | CDC

    E. coli Infection. E. coli normally lives in your intestines. Most strains are usually harmless. A few strains cause diarrhea/bloody diarrhea, vomiting and stomach pains and cramps. One strain can lead to kidney failure if not properly managed. Eating contaminated food is the most common way to get an E. coli …

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  • E. coli: What is It, How Does it Cause Infection,

    2018-3-12 · E. coli is a member of the bacterial family of Enterobacteriaceae [].It is a most common intestinal microorganism of humans and warm-blooded animals [].Some strains of this bacterium have the pathogenic potential and can cause a wide range of infections from mild to life-threatening conditions, such as traveler’s diarrhea, infant diarrhea, hemorrhagic colitis, hemolytic uremic syndrome ...

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  • Voltammetric determination of the Escherichia coli

    2014-2-26 · Symptomatic E coli UTI: presence of typical lower urinary tract symptoms, pyuria (≥8 leukocytes/mm 3), and E coli ≥10 2 CFU/mL in voided midstream urine; recurrent symptomatic UTI: ≥2 episodes within 12 months: ABO blood group secretor status, UTI history: Daily diaries, clinic visits; saliva, blood and urine samples

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  • Urinary Tract Infections in Older Women

    2021-4-13 · Typical features: The colonies of salmonella are purple, E. coli and coliform bacteria are blue, the other bacteria are yellow or colorless. Storage: The prepared plates can be kept for 2-5 days, avoid direct exposure to light. The powder medium should be placed in

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  • How to Detect Microorganisms in Food: Methods and ...

    2018-8-1 · A slide method to detect and enumerate viable cells has been developed. The method employs the use of the tetrazolium salt (p-iodophenyl-3-p-nitrophenyl)-5-phenyl tetrazolium chloride (INT). In this method cells are exposed to filter-sterilized INT for 10 minutes at 37°C in water bath followed by filtration on 0.45-μm membranes.

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  • Assessment of technique for rapid detection of

    E. coli or P. mirabilis mixed with significant numbers of another organism were not detected in four out of five urines. The technique is simple and could be automated. It appears to merit more extensive trial in a hospital laboratory and further development to detect and correctly identify more species that cause urinary tract infections.

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  • COLIKAT RAPID - Enumeration of Escherichia coli and ...

    2012-4-30 · Researchers have developed a rapid testing method using a simple paper strip that can detect E. coli in recreational water within minutes. The new …

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  • Rapid test strips detect bacterial contamination in ...

    2018-11-5 · Electrical engineers have adapted a molecular electronic device called a single-molecule break junction to detect RNA from strains of E. coli known for causing illness.

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  • E. coli in Urine - Causes, Symptoms, Treatment

    2018-3-12 · E. coli is a member of the bacterial family of Enterobacteriaceae [].It is a most common intestinal microorganism of humans and warm-blooded animals [].Some strains of this bacterium have the pathogenic potential and can cause a wide range of infections from mild to life-threatening conditions, such as traveler’s diarrhea, infant diarrhea, hemorrhagic colitis, hemolytic uremic syndrome ...

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  • Detecting E. coli strains using molecular electronics ...

    E. coli Infection. E. coli normally lives in your intestines. Most strains are usually harmless. A few strains cause diarrhea/bloody diarrhea, vomiting and stomach pains and cramps. One strain can lead to kidney failure if not properly managed. Eating contaminated food is the most common way to get an E. coli …

    Get Price
  • E. Coli: Symptoms, Diagnosis, and Treatment

    2021-4-13 · Typical features: The colonies of salmonella are purple, E. coli and coliform bacteria are blue, the other bacteria are yellow or colorless. Storage: The prepared plates can be kept for 2-5 days, avoid direct exposure to light. The powder medium should be placed in

    Get Price
  • Voltammetric determination of the Escherichia coli

    2018-8-1 · A slide method to detect and enumerate viable cells has been developed. The method employs the use of the tetrazolium salt (p-iodophenyl-3-p-nitrophenyl)-5-phenyl tetrazolium chloride (INT). In this method cells are exposed to filter-sterilized INT for 10 minutes at 37°C in water bath followed by filtration on 0.45-μm membranes.

    Get Price
  • Product Catalog

    Features and Benefits: Saves Money Reduce the need for expensive automated ID cards. Easy Read-Out Distinct color reactions for each of the common urinary tract pathogens make it easier to detect mixed infections. Confirmatory Identify E. coli and Enterococcus spp. with no further testing needed.

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  • A Novel Detection of Enterococcus faecalis Using

    A Novel Detection of Enterococcus faecalis Using Multiple Cross Displacement Amplification Linked with Gold Nanoparticle Lateral Flow Biosensor Xu Chen,1,2 Kai Ma,3 Xu Yi,1 Ziyu Xiao,4 Lijuan Xiong,3 Yu Wang,5 Shijun Li2 1Central Laboratory of the Second Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550003, People’s Republic of China; 2Laboratory ...

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  • Microfluidic Capillaric Circuit for Rapid and Facile ...

    2017-5-25 · Up to 50% of women will get a urinary tract infection (UTI) by age 32. UTI is caused by the presence of bacteria in urine, with over 80% of cases caused by E. coli. The gold standard for detecting bacteria in urine is plate culture and the traditional clinical diagnostic threshold is ≥10 5 colony-forming units per mL (CFU/mL). However, plate culture is slow (24–72 h to provide results ...

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  • In Silico Analysis for Determination and Validation of ...

    2018-12-15 · Avian pathogenic Escherichia coli (APEC), a subgroup of extra-intestinal pathogenic E. coli (ExPEC) causes avian coli bacillosis and burden economic losses in the global poultry industry (Singer 2015).However, the APEC pathogenesis is not well known. Many of the virulence genes have been studied in order to identify viral agents in the APEC, consist of those that have been studied in …

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  • Pyruvate Assay Kit (ab65342) | Abcam

    2021-4-6 · Product number Product name Product specification Directions HB0114-1 VRB-MUG Agar VRB-MUG琼脂 100g Used for solid plate detection for E. coli (GB standard) HB0141 Aliz-gal Agar 茜素-β-半乳糖苷琼脂(Aliz-gal琼脂) 100g For the rapid detect and count

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  • CRISPR-Based Diagnosis of Infectious and

    Uric acid in urine is going to strongly interfere with the assay so besides doing the normal standard curve, the lab recommends to also do standard curve in the presence of X volume of diluted urine (may be 4-15 ul depends). Due to the presence of uric acid in the urine, the slope of standard curve with the urine …

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  • Imaging, Identification and Inhibition of

    2020-9-14 · Since the discovery of the clustered regularly interspaced short palindromic repeats (CRISPR) locus in Escherichia coli (E. coli) in 1987, [] CRISPR has revolutionized both research and practical achievements in biology, particularly in the areas of genome editing and genetic engineering.CRISPR-associated genes (cas genes) were identified in 2002 [] and further research has …

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  • How to Detect Microorganisms in Food: Methods and ...

    2021-4-9 · Microorganisms, including bacteria, viruses and fungi, are ubiquitous in nature. Some are extremely beneficial to life on Earth, whereas some cause diseases and disrupt normal human physiology. Pathogenic microorganisms can also undergo mutations and develop resistance to antimicrobial agents, which complicates diagnostic and therapeutic regimens. This calls for continuing …

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  • E. coli (Escherichia coli) | E. coli | CDC

    Escherichia coli (abbreviated as E. coli) are bacteria found in the environment, foods, and intestines of people and animals.E. coli are a large and diverse group of bacteria. Although most strains of E. coli are harmless, others can make you sick. Some kinds of E. coli can cause diarrhea, while others cause urinary tract infections, respiratory illness and pneumonia, and other illnesses.

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  • Legionella Urine Test, Legionella Urinary Antigen Test

    2016-5-18 · Legionella Urine ELISA kit description: This Legionella Urinary Antigen test is intended as an adjunct to culture for the presumptive diagnosis of past or current Legionnaires disease by qualitative detection of Legionella pneumophilia antigen in human urine. 1.Plate: Microwells containing purified anti- Legionella pneumophilia antibodies.

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  • Proteomics and metabolomics analysis reveal

    2020-7-17 · 1. Introduction E. coli (Escherichia coli) has the characteristics of being both a widespread gut commensal of vertebrates and a versatile pathogen. 1 E. coli is a common cause of urinary tract infection and intra-abdominal infection in humans of all ages, 2 and it kills more than 2 million humans annually in the form of intra- and extra-intestinal diseases. 3,4 ESBLs (Extended-spectrum β ...

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  • Oral fosfomycin for treatment of urinary tract infection ...

    2017-8-25 · colorimetric redox indicator to detect active growth of an organism in the presence of the antimicrobial. The organism to be tested is grown on a non-selective medium in appropriate conditions (37 °C in O 2 for E. coli Klebsiella spp.) for 16–18 h, before a 0.5 MacFar-land suspension is prepared (BD AutoPrep). This sus-

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  • Oral fosfomycin for treatment of urinary tract

    2016-10-11 · The majority had urine cultures positive for E. coli (52/75, 69 %; Table 2). ESBL-production was reported in 31/52 of the E. coli isolates (59 %) and 6/9 K. pneumoniae (67 %); (Table 2 ). Overall, therefore, only 31/75 (41 %) of all isolates met the microbiological criterion (ESBL E. coli ) for fosfomycin treatment stipulated by NICE guidance ...

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  • Structure-based discovery of glycomimetic FmlH

    2018-3-20 · FmlH residues 1 to 160 from UPEC strain UTI89 with a C-terminal six-histidine tag (i.e., FmlH LD) were cloned into the IPTG-inducible plasmid pTrc99A. This construct was then transformed into and expressed in E. coli strain C600. Periplasms were isolated as previously described and dialyzed four times against PBS plus 250 mM NaCl .

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  • Imaging, Identification and Inhibition of

    2021-4-6 · Product number Product name Product specification Directions HB0114-1 VRB-MUG Agar VRB-MUG琼脂 100g Used for solid plate detection for E. coli (GB standard) HB0141 Aliz-gal Agar 茜素-β-半乳糖苷琼脂(Aliz-gal琼脂) 100g For the rapid detect and count

    Get Price
  • Cytological Diagnosis of Infectious Diseases ...

    2021-4-9 · Microorganisms, including bacteria, viruses and fungi, are ubiquitous in nature. Some are extremely beneficial to life on Earth, whereas some cause diseases and disrupt normal human physiology. Pathogenic microorganisms can also undergo mutations and develop resistance to antimicrobial agents, which complicates diagnostic and therapeutic regimens. This calls for continuing …

    Get Price
  • CRISPR-Based Diagnosis of Infectious and

    2020-9-14 · Since the discovery of the clustered regularly interspaced short palindromic repeats (CRISPR) locus in Escherichia coli (E. coli) in 1987, [] CRISPR has revolutionized both research and practical achievements in biology, particularly in the areas of genome editing and genetic engineering.CRISPR-associated genes (cas genes) were identified in 2002 [] and further research has …

    Get Price
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